DNA intercalator stimulates influenza transcription and virus replication

DNA intercalator stimulates influenza transcription and virus replication

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Abstract Influenza A virus uses its host transcription machinery to facilitate viral RNA synthesis, an event that is associated with cellular RNA polymerase II (RNAPII).In this study, various RNAPII transcription inhibitors were used to investigate the effect of RNAPII phosphorylation status on viral click here RNA transcription.A low concentration of DNA intercalators, such as actinomycin D (ActD), was found to stimulate viral polymerase activity and virus replication.

This effect was not observed in cells treated with RNAPII kinase 355 maybelline fit me inhibitors.In addition, the loss of RNAPIIa in infected cells was due to the shift of nonphosphorylated RNAPII (RNAPIIa) to hyperphosphorylated RNAPII (RNAPIIo).